2017-06-23 06:48:542025-05-02 18:05:45
locus
BSU22750
BSU_22750
geneLength
699
702
outlinks
bsu
BSU22750
BSU_22750
Gene
Phenotypes of a mutant
essential [http://www.ncbi.nlm.nih.gov/pubmed/?term=12682299 according to Kobayashi et al. ]; however, inactivation reported by [http://www.ncbi.nlm.nih.gov/pubmed/?term=26735940 Meeske et al. ] and [http://www.ncbi.nlm.nih.gov/pubmed/?term=20511502 Block et al. ]
inactivation of ''[[gene|menH]]'' reduces sporulation efficiency to 12.7% that of wild type cells [Pubmed|26735940]
essential [http://www.ncbi.nlm.nih.gov/pubmed/?term=12682299 according to Kobayashi et al.]; however, inactivation reported by [http://www.ncbi.nlm.nih.gov/pubmed/?term=26735940 Meeske et al.] and [http://www.ncbi.nlm.nih.gov/pubmed/?term=20511502 Block et al.]
inactivation of ''[[gene|menH]]'' reduces sporulation efficiency to 12.7% that of wild type cells [Pubmed|26735940]
defective in biofilm formation [pubmed|31420537]
The protein
Catalyzed reaction/ biological activity
S-adenosyl-L-methionine + demethylmenaquinol = s-adenosyl-L-homocysteine + menaquinol (according to Swiss-Prot)
2-demethylmenaquinol + S-adenosyl-L-methionine --> menaquinol + H+ + S-adenosyl-L-homocysteine (according to UniProt)
Biological materials
Mutant
BKE22750 (menH::erm trpC2) available at the [http://bgsc.org Bacillus Genetic Stock Center]
BKK22750 (menH::kan trpC2) available at the [http://bgsc.org Bacillus Genetic Stock Center]
BKE22750 (Δ[[gene|menH]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCTTTTGAGTCCTGCATAA, downstream forward: _UP4_CTGTTTGAAGAGGCGGGCCT
BKK22750 (Δ[[gene|menH]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCTTTTGAGTCCTGCATAA, downstream forward: _UP4_CTGTTTGAAGAGGCGGGCCT
References
The protein
Protein family
[SW|Methyltransferase superfamily] (according to UniProt)
[SW|class I-like SAM-binding methyltransferase superfamily] (according to UniProt)
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
[SW|AB hydrolase-1 domain] (aa 26-259) (according to InterPro)
Expression and Regulation
additional information
highly expressed at high iron concentrations [pubmed|31420537]